In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 82
... medium containing nerve growth factor ( NGF ) , they rapidly differentiate from a spherical , chromaffin - like cell to a neuron - like cell with neurites and other neuronal markers ( 10 ) . These proper- ties make this cell line the ...
... medium containing nerve growth factor ( NGF ) , they rapidly differentiate from a spherical , chromaffin - like cell to a neuron - like cell with neurites and other neuronal markers ( 10 ) . These proper- ties make this cell line the ...
Pagina 83
Charles A. Tyson, John M. Frazier. Double - strength growth medium : RPMI 1640 medium supplemented with ( v / v ) 20 % heat - inactivated equine serum , 10 % fetal calf serum , and 1 % penicillin - streptomycin as described for growth ...
Charles A. Tyson, John M. Frazier. Double - strength growth medium : RPMI 1640 medium supplemented with ( v / v ) 20 % heat - inactivated equine serum , 10 % fetal calf serum , and 1 % penicillin - streptomycin as described for growth ...
Pagina 84
... growth medium and added to an equal volume of a 1 % solution of agar which has been melted and cooled to 40 ° C . The inoculum is then quickly mixed and overlaid on a petri dish that had been previously layered with 1 % agar base . Cell ...
... growth medium and added to an equal volume of a 1 % solution of agar which has been melted and cooled to 40 ° C . The inoculum is then quickly mixed and overlaid on a petri dish that had been previously layered with 1 % agar base . Cell ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml