In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 1
Charles A. Tyson, John M. Frazier. 1 Hippocampal Slices Peter G. Aitken INTRODUCTION Pioneered in the early 1970s by Skrede and Westgaard ( 1 ) , the in vitro hippocampal slice has since become a widely used preparation for studies of ...
Charles A. Tyson, John M. Frazier. 1 Hippocampal Slices Peter G. Aitken INTRODUCTION Pioneered in the early 1970s by Skrede and Westgaard ( 1 ) , the in vitro hippocampal slice has since become a widely used preparation for studies of ...
Pagina 3
... hippocampal slice maintenance . Many different designs have been used successfully , including both commercial units and homemade designs . Design specifics depend to some extent on the experiments being planned . For example ...
... hippocampal slice maintenance . Many different designs have been used successfully , including both commercial units and homemade designs . Design specifics depend to some extent on the experiments being planned . For example ...
Pagina 5
... hippocampus embedded in other tissue . These slices can be used as is , or the hippocampal portion can be dissected free . This and other microdissection pro- cedures ( such as isolating certain regions of the hippocampus ) can be ...
... hippocampus embedded in other tissue . These slices can be used as is , or the hippocampal portion can be dissected free . This and other microdissection pro- cedures ( such as isolating certain regions of the hippocampus ) can be ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml