In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 151
... incubator ( Forma Scientific ) . The incubator is maintained at 37 ° C , and a pH of 7.2 to 7.4 is regulated by a mixture of 5 % CO2 and 95 % air ( v / v ) . Depending on the number of cells needed for toxicologic studies ...
... incubator ( Forma Scientific ) . The incubator is maintained at 37 ° C , and a pH of 7.2 to 7.4 is regulated by a mixture of 5 % CO2 and 95 % air ( v / v ) . Depending on the number of cells needed for toxicologic studies ...
Pagina 248
... incubator at 37 ° C . DMEM Solution 1. Warm overnight two 90 - ml aliquots of culture medium to 37 ° C in the CO2 incubator . 2. Thaw two 10 - ml aliquots of serum / antibiotics solution ( ~ 30 min before use ) . 3. Add the 10 - ml ...
... incubator at 37 ° C . DMEM Solution 1. Warm overnight two 90 - ml aliquots of culture medium to 37 ° C in the CO2 incubator . 2. Thaw two 10 - ml aliquots of serum / antibiotics solution ( ~ 30 min before use ) . 3. Add the 10 - ml ...
Pagina 421
... incubator at 37 ° C . A hole is drilled in the side of the incubator , the motor shaft is inserted , and a connection made to a rotator wheel with clips to hold the culture bottles ( Fig . 1 ) . Figure 1 Frontal view of an incubator ...
... incubator at 37 ° C . A hole is drilled in the side of the incubator , the motor shaft is inserted , and a connection made to a rotator wheel with clips to hold the culture bottles ( Fig . 1 ) . Figure 1 Frontal view of an incubator ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
Parole e frasi comuni
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml