In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Risultati 1-3 di 71
Pagina 352
... kidneys toward and under the liver . Leave the fat tissue surrounding the kidneys but cut away the gut overlying the left kidney . Continue trimming until the kidneys are free . Trim away any excess liver above the ligature that is ...
... kidneys toward and under the liver . Leave the fat tissue surrounding the kidneys but cut away the gut overlying the left kidney . Continue trimming until the kidneys are free . Trim away any excess liver above the ligature that is ...
Pagina 353
... kidneys to the funnel recirculating apparatus . Monitor the progress of the digestion by firmly pressing on the kidneys with a sterile swab . When the tissue is soft and breaks apart to the touch it is sufficiently di- gested . For ...
... kidneys to the funnel recirculating apparatus . Monitor the progress of the digestion by firmly pressing on the kidneys with a sterile swab . When the tissue is soft and breaks apart to the touch it is sufficiently di- gested . For ...
Pagina 369
... kidneys with renal arteries and veins intact are removed . The kidneys are placed in a sterile 50 - ml tube containing DME / F12 ( at 4 ° C ) with 192 IU / ml penicillin and 200 μg / ml streptomycin . Tubes containing the kidneys are ...
... kidneys with renal arteries and veins intact are removed . The kidneys are placed in a sterile 50 - ml tube containing DME / F12 ( at 4 ° C ) with 192 IU / ml penicillin and 200 μg / ml streptomycin . Tubes containing the kidneys are ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
Parole e frasi comuni
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml