In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 136
... labeling index ( LI ) , cultures are exposed for 24 hr to [ 3H ] thymidine . After the labeling period the culture media are re- moved , and the cultures are washed with PBS and then dissociated by trypsin - EDTA treatment ...
... labeling index ( LI ) , cultures are exposed for 24 hr to [ 3H ] thymidine . After the labeling period the culture media are re- moved , and the cultures are washed with PBS and then dissociated by trypsin - EDTA treatment ...
Pagina 137
... labeled . Labeling indices are determined by scoring 1000 cells . Factors Affecting the Growth of Rat Tracheal Epithelial Cell Cultures There are two assays routinely used to assess growth of RTE cells in culture . The first is the ...
... labeled . Labeling indices are determined by scoring 1000 cells . Factors Affecting the Growth of Rat Tracheal Epithelial Cell Cultures There are two assays routinely used to assess growth of RTE cells in culture . The first is the ...
Pagina 547
... Labeling index , thymidine , 136–137 B - Lactam antibiotics , 407 Lactate dehydrogenase efflux , for assessment of neurotoxic damage , 53 leakage assay for assessment of interstitial cell vi- ability in culture , 387 for assessment of ...
... Labeling index , thymidine , 136–137 B - Lactam antibiotics , 407 Lactate dehydrogenase efflux , for assessment of neurotoxic damage , 53 leakage assay for assessment of interstitial cell vi- ability in culture , 387 for assessment of ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml