In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 113
... macrophages will ingest particles , thus making the separation by density of macrophages and type II cells incomplete . One must also carefully choose an appropriate gradient material . It is impor- tant to determine if the material ...
... macrophages will ingest particles , thus making the separation by density of macrophages and type II cells incomplete . One must also carefully choose an appropriate gradient material . It is impor- tant to determine if the material ...
Pagina 455
... macrophages are found in most every other organ / tissue including the liver ( Kupffer cells ) , lung ( alveolar macrophages ) , skin ( Langerhans cells ) , and brain . As summarized in Table I , these cells participate in immune ...
... macrophages are found in most every other organ / tissue including the liver ( Kupffer cells ) , lung ( alveolar macrophages ) , skin ( Langerhans cells ) , and brain . As summarized in Table I , these cells participate in immune ...
Pagina 464
... macrophages . In fact , the in vivo environment for many tissue macrophage populations is devoid of serum proteins . The addition of serum to macrophage cultures often en- hances certain activities . Considering the potent activating ...
... macrophages . In fact , the in vivo environment for many tissue macrophage populations is devoid of serum proteins . The addition of serum to macrophage cultures often en- hances certain activities . Considering the potent activating ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml