In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 282
... mg 240 mg Dissolve into 31 ml sterile distilled water Dispense amino acid stock solution into 1.55 - ml aliquots and freeze at -20 ° C . Can be stored for 6 months . AB basal medium 10 × Waymouth's MB 752/1 ( GIBCO ) 50 ml 1.55 ml 1.0 ...
... mg 240 mg Dissolve into 31 ml sterile distilled water Dispense amino acid stock solution into 1.55 - ml aliquots and freeze at -20 ° C . Can be stored for 6 months . AB basal medium 10 × Waymouth's MB 752/1 ( GIBCO ) 50 ml 1.55 ml 1.0 ...
Pagina 445
... ml MEM Insulin Transferrin Selenous acid 0.625 mg / ml 0.625 mg / ml 6.25 μg / ml 6.25 μg / ml 0.625 μg / ml 6.25 ng / ml Bovine serum albumin ( BSA ) 125 mg / ml 1.25 mg / ml Linoleic acid 0.535 mg / ml 5.35 μg / ml Retinol acetate ...
... ml MEM Insulin Transferrin Selenous acid 0.625 mg / ml 0.625 mg / ml 6.25 μg / ml 6.25 μg / ml 0.625 μg / ml 6.25 ng / ml Bovine serum albumin ( BSA ) 125 mg / ml 1.25 mg / ml Linoleic acid 0.535 mg / ml 5.35 μg / ml Retinol acetate ...
Pagina 495
... ml of fresh medium . On days 8-10 , cultures are rinsed with phosphate - buffered saline ( PBS ) , allowed to air ... mg / ml ) 1.0 ml glucose ( 540 mg / ml ) ( Sigma , No. G 1392 ) 0.2 ml glutamine ( Sigma , No. G 1517 ) ( 164 mg / ml ) ...
... ml of fresh medium . On days 8-10 , cultures are rinsed with phosphate - buffered saline ( PBS ) , allowed to air ... mg / ml ) 1.0 ml glucose ( 540 mg / ml ) ( Sigma , No. G 1392 ) 0.2 ml glutamine ( Sigma , No. G 1517 ) ( 164 mg / ml ) ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml