In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 120
... pellet in 4-8 ml of warm DME / F12 medium without serum . Filter through a piece of 160 - μm nylon mesh to remove any clumps . 26. Perform cell count to determine final yield . Culture of Type II Cells 1. Resuspend pellet of type II ...
... pellet in 4-8 ml of warm DME / F12 medium without serum . Filter through a piece of 160 - μm nylon mesh to remove any clumps . 26. Perform cell count to determine final yield . Culture of Type II Cells 1. Resuspend pellet of type II ...
Pagina 298
... pellet in 40 ml fresh MEM with 1 ml DNase solution . A band of lipocytes can be recognized at the top of each pellet as a white layer of cells . 5. During the cell washes prepare four larex gradient tubes as follows : a . Sterilize the ...
... pellet in 40 ml fresh MEM with 1 ml DNase solution . A band of lipocytes can be recognized at the top of each pellet as a white layer of cells . 5. During the cell washes prepare four larex gradient tubes as follows : a . Sterilize the ...
Pagina 461
... pellet is then washed three times in Gey's BSS . To separate cells the pellet is resuspended in 80 ml of 50 % Percoll and centrifuged at 27,000 g at 22 ° C for 30 min , after which cells are collected from the middle zone of the ...
... pellet is then washed three times in Gey's BSS . To separate cells the pellet is resuspended in 80 ml of 50 % Percoll and centrifuged at 27,000 g at 22 ° C for 30 min , after which cells are collected from the middle zone of the ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml