In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 17
... pipette , and the distal 0.5 mm closest to the operator is removed by crosscut- ting with a new pair of scalpel blades whose tips have been dipped in nutrient medium for lubrication . The cerebellum is then cross cut into seven or eight ...
... pipette , and the distal 0.5 mm closest to the operator is removed by crosscut- ting with a new pair of scalpel blades whose tips have been dipped in nutrient medium for lubrication . The cerebellum is then cross cut into seven or eight ...
Pagina 51
... pipette . Incubate the tissue for 1 hr in a 37 ° C water bath . Plating Mixed Cortical Neuronal and Glial Cells 1. Remove the cells from the water bath and place them in a clinical cen- trifuge for 5 min ( ~ 1000 g ) . 2. Take two ...
... pipette . Incubate the tissue for 1 hr in a 37 ° C water bath . Plating Mixed Cortical Neuronal and Glial Cells 1. Remove the cells from the water bath and place them in a clinical cen- trifuge for 5 min ( ~ 1000 g ) . 2. Take two ...
Pagina 298
... pipette . 7. Layer the 25 ml of cell suspension evenly across the four gradient tubes using a sterile transfer pipette . 8. Centrifuge the gradients in an SW - 40 rotor at 20,000 rpm for 25 min at 25 ° C in a Beckman ultracentrifuge ...
... pipette . 7. Layer the 25 ml of cell suspension evenly across the four gradient tubes using a sterile transfer pipette . 8. Centrifuge the gradients in an SW - 40 rotor at 20,000 rpm for 25 min at 25 ° C in a Beckman ultracentrifuge ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml