In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 344
... protein determination . One hundred mi- croliters of the tubule suspension is solubilized in 100 μl of 0.4 % Triton X - 100 in 80 mM NaOH , and protein is measured spectrophotometrically as described by Bradford ( 11 ) . The standard ...
... protein determination . One hundred mi- croliters of the tubule suspension is solubilized in 100 μl of 0.4 % Triton X - 100 in 80 mM NaOH , and protein is measured spectrophotometrically as described by Bradford ( 11 ) . The standard ...
Pagina 363
... Protein Recoveries " Percent Percoll Tubular density limits ( g / ml ) Hexokinase ( distal ) Leucine aminopeptidase ( proximal ) Protein recovery ( mg ) Cortex cortices ( for PCT isolation ) FT 40 < 1.053 45 1.054-1.059 13.1 ± 0.9 22.02 ...
... Protein Recoveries " Percent Percoll Tubular density limits ( g / ml ) Hexokinase ( distal ) Leucine aminopeptidase ( proximal ) Protein recovery ( mg ) Cortex cortices ( for PCT isolation ) FT 40 < 1.053 45 1.054-1.059 13.1 ± 0.9 22.02 ...
Pagina 486
... protein A - Sepharose column to remove IgG that would interfere with the reverse plaque assay ( 8 ) . The column is prepared by pipetting 2 ml of protein A - Sepharose ( Sigma ) into a 10 - ml disposable column ( Bio - Rad , Richmond ...
... protein A - Sepharose column to remove IgG that would interfere with the reverse plaque assay ( 8 ) . The column is prepared by pipetting 2 ml of protein A - Sepharose ( Sigma ) into a 10 - ml disposable column ( Bio - Rad , Richmond ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml