In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 343
... tubule yields . The two most critical factors in the dissociation process are the disease state of the kidneys and the collagenase . As discussed above , the use of SPF animals helps to ensure healthy kidneys ... Proximal Tubule Culture 343.
... tubule yields . The two most critical factors in the dissociation process are the disease state of the kidneys and the collagenase . As discussed above , the use of SPF animals helps to ensure healthy kidneys ... Proximal Tubule Culture 343.
Pagina 345
... proximal tubules in short - term primary culture . Preparation time is mini- mal ( ~ 3 hr ) , and the high tubule yields ( ~ 200 mg protein per rabbit ) provide adequate tissue for dose - response and time course studies . Suspension ...
... proximal tubules in short - term primary culture . Preparation time is mini- mal ( ~ 3 hr ) , and the high tubule yields ( ~ 200 mg protein per rabbit ) provide adequate tissue for dose - response and time course studies . Suspension ...
Pagina 371
... proximal tubule cells is highly reproducible and yields a large number of cells . As many as 100 35 - mm dishes containing confluent monolayers can be obtained from a single rabbit kidney . Moreover the cultures express functional ...
... proximal tubule cells is highly reproducible and yields a large number of cells . As many as 100 35 - mm dishes containing confluent monolayers can be obtained from a single rabbit kidney . Moreover the cultures express functional ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml