In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina viii
... Renal Proximal Tubules 339 Kathleen G. Dickman and Lazaro J. Mandel 31. Perfusion Method for Isolation of Renal Proximal Tubules from Mammalian Species Jack E. Dabbs , Carol E. Green , and Charles A. Tyson 348 32. Isolation and ...
... Renal Proximal Tubules 339 Kathleen G. Dickman and Lazaro J. Mandel 31. Perfusion Method for Isolation of Renal Proximal Tubules from Mammalian Species Jack E. Dabbs , Carol E. Green , and Charles A. Tyson 348 32. Isolation and ...
Pagina 339
... renal cell types . To circumvent these problems , a variety of preparations are available for in vitro nephrotoxicity research including kidney slices , isolated renal tubules and cells from defined nephron segments , and isolated ...
... renal cell types . To circumvent these problems , a variety of preparations are available for in vitro nephrotoxicity research including kidney slices , isolated renal tubules and cells from defined nephron segments , and isolated ...
Pagina 558
... renal cell lines derived from , 411-419 . See also LLC - RK1 ; Renal cell lines , derived from rabbit and pig kidney for primary kidney cell cultures , 368 for primary proximal tubule culture , 341 renal cortex , isolation of , 342 renal ...
... renal cell lines derived from , 411-419 . See also LLC - RK1 ; Renal cell lines , derived from rabbit and pig kidney for primary kidney cell cultures , 368 for primary proximal tubule culture , 341 renal cortex , isolation of , 342 renal ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml