In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 152
... studies are unethical to conduct in humans . Traditionally , whole - animal toxicologic studies have been used to investigate chemical injury to the host organism ( 2 ) . Although the data are useful and indicate actual in vivo ...
... studies are unethical to conduct in humans . Traditionally , whole - animal toxicologic studies have been used to investigate chemical injury to the host organism ( 2 ) . Although the data are useful and indicate actual in vivo ...
Pagina 339
... studies provide the most definitive information regarding toxicity of a test compound , mecha- nistic studies are complicated in the in vivo setting by hemodynamic effects , in- terorgan metabolite conversion , and the heterogeneity of ...
... studies provide the most definitive information regarding toxicity of a test compound , mecha- nistic studies are complicated in the in vivo setting by hemodynamic effects , in- terorgan metabolite conversion , and the heterogeneity of ...
Pagina 522
... Studies also have been conducted examining the process of transdermal drug delivery by iontophoresis ( 12,13 ) . In these studies , transdermal flux of lidocaine could be changed by coiontophoresing vasoactive drugs , a finding not seen ...
... Studies also have been conducted examining the process of transdermal drug delivery by iontophoresis ( 12,13 ) . In these studies , transdermal flux of lidocaine could be changed by coiontophoresing vasoactive drugs , a finding not seen ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml