In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 414
... Viability measurements using these probes are conducted in situ , and viability can therefore be measured either as a terminal end point or continuously . Following the incubation of the test agent , the cultures are rinsed one time ...
... Viability measurements using these probes are conducted in situ , and viability can therefore be measured either as a terminal end point or continuously . Following the incubation of the test agent , the cultures are rinsed one time ...
Pagina 417
... viability , they found that the basolateral surface of LLC - PK1 cells was more sensitive to drug exposure than the brush border surface . Second , they found that , for several drugs , the in situ measurement of viability by ...
... viability , they found that the basolateral surface of LLC - PK1 cells was more sensitive to drug exposure than the brush border surface . Second , they found that , for several drugs , the in situ measurement of viability by ...
Pagina 504
... viability in penetration experiments has not been resolved . When using excised human skin ( surgical or cadaver ) there is usually decreased tissue viability because of the lag time between collection of tissue and the beginning of the ...
... viability in penetration experiments has not been resolved . When using excised human skin ( surgical or cadaver ) there is usually decreased tissue viability because of the lag time between collection of tissue and the beginning of the ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml