In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 197
... Viral or bacte- rial infection can be a difficult problem because the gastrointestinal system is sensitive to infections , and gastrointestinal symptoms can precede other signs of infection . Diagnosis is frequently difficult , and ...
... Viral or bacte- rial infection can be a difficult problem because the gastrointestinal system is sensitive to infections , and gastrointestinal symptoms can precede other signs of infection . Diagnosis is frequently difficult , and ...
Pagina 490
... virus ( HIV ) and treated accordingly . Institutional approval may be required from the appropriate committees before blood can be drawn from subjects in the laboratory . ACKNOWLEDGMENTS We would like to acknowledge Mr. James Karras and ...
... virus ( HIV ) and treated accordingly . Institutional approval may be required from the appropriate committees before blood can be drawn from subjects in the laboratory . ACKNOWLEDGMENTS We would like to acknowledge Mr. James Karras and ...
Pagina 545
... virus , 490 Human liver specimens dissociation of , conditions for , 272 , 274 for hepatocyte isolation , 268 source of , 265 Human peripheral blood lymphocytes , 484 Human placental serum , 15 Human renal proximal tubules , isolation ...
... virus , 490 Human liver specimens dissociation of , conditions for , 272 , 274 for hepatocyte isolation , 268 source of , 265 Human peripheral blood lymphocytes , 484 Human placental serum , 15 Human renal proximal tubules , isolation ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml