GB 5009.208-2016: Translated English of Chinese Standard. GB5009.208-2016: National Food Safety Standard - Determination of Biogenic Amine in Food
https://www.chinesestandard.net, 14 set 2019 - 17 pagine
This Standard specifies the determination method for tryptamine, -phenylethylamine, putrescine, cadaverine, histamine, octopamine, tyramine, spermidine and spermine content in food. This Standard is applicable to the determination of alcohol (wine, beer and yellow rice wine, etc.), condiments (vinegar and soy sauce), aquatic products (fish and its products; shrimp and its products), biogenic amine in meat.
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Instruments and Equipment
Expression of Analytical Result
Appendix A Liquid Chromatogram of 9 Biogenic Amine Standard Solutions and Internal Standard Derivatives
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0.01 mol/L ammonium 0.1 mol/L hydrochloric 40 C water absorb accurately weigh-take acetic acid Add 1 mL Alcohol amount of water aquatic products biogenic amine standard cadaverine calculated chromatographically column completely dissolve concentration conduct vortex oscillation constant volume Dansyl chloride derivation derivatization detection Determination of Biogenic ether expressed in mL extract filter Food g of sodium GB/T histamine hydrochloric acid solution internal standard Method mg/mL mL beaker mL of hydrochloric mL volumetric flask Mobile phase mol/L ammonium acetate mol/L hydrochloric acid months obtained octopamine plug Preparation products and meat proper amount putrescine reach a constant Reagents remove Reserve respectively Result right before usage sample solution scale shelf sodium chloride sodium glutamate sodium hydroxide solution solution 100 mg/L spermidine spermine standard solution standard stock solutions standard substance purity standard working solution stratification Take transfer trichloroacetic acid solution Trichloromethane tryptamine tyramine vinegar water bath water to completely