Laboratory Procedures in Clinical MicrobiologyJ.A. Washington Springer Science & Business Media, 6 dic 2012 - 885 pagine Although there are a number of comprehensive books in clinical micro biology, there remains a need for a manual that can be used in the clinical laboratory to guide the daily performance of its work. Most of the existing publications provide detailed and precise information, for example, by which a microorganism can be characterized and identified beyond any doubt; however, the number of tests involved in this process exceeds the capabilities and resources of most clinical laboratories and are irrele vant for patient care. It is, therefore, necessary in any clinical laboratory to extract from reference manuals, textbooks, and journals those tests and procedures that are to be used to complete the daily workload as efficiently and accurately as possible. It is also essential in the clinical laboratory to determine, on the basis of the kind of specimen being exam ined, which microorganisms are clinically relevant and require isolation and identification and which should either be excluded selectively or simply regarded as indigenous flora and, therefore, not specifically identi fied. Cost and time limit a laboratory's resources, and priorities must be established for handling the workload. The procedures described in the second edition of this manual are those selected by our staff for use in the clinical laboratory on the basis of clinic~l relevance, accuracy, reproducibility, and efficiency. Alternative procedures, when considered equivalent on the basis of personal or pub lished experience, have been included where appropriate. |
Sommario
24 | |
References | 67 |
III | 103 |
V | 110 |
Cultures of Integumentary Infections | 119 |
1 General Classification of Bacteria | 127 |
GramNegative Cocci | 157 |
GramPositive Bacilli | 164 |
References | 514 |
Identification | 521 |
References | 532 |
Processing of Specimens and Cultures | 539 |
Identification of DNAContaining Viruses | 585 |
Reporting of Viral Isolates | 611 |
Parasites | 625 |
Procedures Suggested for Use in Examination of Clinical | 634 |
GramNegative Bacilli | 179 |
References | 241 |
3 Fluorescent Antibody Procedures | 251 |
Counterimmunoelectrophoresis | 270 |
References | 278 |
Preparation of Antimicrobial Solutions | 288 |
References | 310 |
Isolation of Anaerobic Bacteria | 321 |
Identification of Anaerobic Bacteria | 330 |
Antimicrobial Susceptibility Testing of Anaerobic Bacteria | 373 |
Mycobacteria and Nocardia | 379 |
Antimicrobial Susceptibility Testing | 408 |
References | 417 |
Identification of Yeasts | 426 |
Definitive Tests | 440 |
Identification of Filamentous Fungi | 446 |
Serodiagnosis of Fungal Infections | 487 |
References | 497 |
Cultures for Chlamydia | 503 |
Examination for Blood and Tissue Parasites | 659 |
ས Examination of Specimens Other Than Feces | 669 |
Miscellaneous Procedures | 676 |
References | 685 |
Bioassays | 695 |
Liquid Chromatographic Assays | 709 |
References | 726 |
2 Bactericidal Tests | 731 |
Combination Studies | 739 |
Appendix A Laboratory Safety | 747 |
Environmental Control | 763 |
Media for Aerobic and Facultatively Anaerobic Bacteria | 770 |
Media and Reagents for Anaerobic Bacteria | 800 |
Media and Reagents for Mycobacteria and Nocardia | 808 |
Media Reagents and Buffers for Chlamydiae | 816 |
829 | |
Equipment | 861 |
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Parole e frasi comuni
acid aerobic amikacin anaerobic bacteria antibiotic antibody antigen antimicrobial antiserum assay bacteremia Bacteroides blood agar buffer Candida carbohydrate catalase cell cultures centrifuge Chlamydia Clin clinical specimens Clostridium cocci color concentration containing Cryptococcus neoformans cysts detect diagnosis Difco Laboratories differential dilution disease disk Distilled water Distilled water Final echovirus Enterobacteriaceae Escherichia coli examined fermentation Figure fluid fluorescence fragilis fungi gentamicin glucose gram-negative gram-positive growth Haemophilus Haemophilus influenzae hyphae identification incubation infection influenzae inoculated inoculum Isolated colonies Legionella medium method Microbiol microscopic morphology Motility mycobacteria Mycoplasma NaCl Negative test Neisseria nitrate organisms patients penicillin petri dish plate pneumoniae Positive test prepared Procedure produce Pseudomonas Reagents serological serum slant slide smear Sodium solution species stain Staphylococcus Sterilize at 121°C Streptococcus subculture susceptibility testing suspension swab Table tion tissue titer trachomatis urease urine usually vancomycin vial viral virus viruses water Final pH yeasts µg/ml