In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 12
... rinsing and soaking in deionized wa- ter , the glassware is transferred to stainless steel utility tanks containing 8 ... rinse cycle of the laboratory dishwasher . The glassware is oven dried and , after cooling , wrapped as necessary ...
... rinsing and soaking in deionized wa- ter , the glassware is transferred to stainless steel utility tanks containing 8 ... rinse cycle of the laboratory dishwasher . The glassware is oven dried and , after cooling , wrapped as necessary ...
Pagina 64
... rinse . After the rinse , water is replaced with 10 ml of 1 N HCl , and the flask is covered with fresh Parafilm . The coverslips are exposed to ultrasonication for another 20 min . The acid is drained and the coverslips rinsed again ...
... rinse . After the rinse , water is replaced with 10 ml of 1 N HCl , and the flask is covered with fresh Parafilm . The coverslips are exposed to ultrasonication for another 20 min . The acid is drained and the coverslips rinsed again ...
Pagina 472
... rinse the columns with 100 ml of 37 ° C complete medium . Close the stopcock , add sufficient medium to completely cover the nylon wool , and eliminate air bubbles from the column . These procedures should be conducted aseptically in a ...
... rinse the columns with 100 ml of 37 ° C complete medium . Close the stopcock , add sufficient medium to completely cover the nylon wool , and eliminate air bubbles from the column . These procedures should be conducted aseptically in a ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
Parole e frasi comuni
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml