In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 233
... cannula unit ; ( 4 ) liver support dish ; ( 5 ) perfusate reservoir . withstand a certain pressure , and the filter material should not clog . Our version consists of a 5 - cm piece of 8 × 10 mm silicone rubber tubing , stoppered with ...
... cannula unit ; ( 4 ) liver support dish ; ( 5 ) perfusate reservoir . withstand a certain pressure , and the filter material should not clog . Our version consists of a 5 - cm piece of 8 × 10 mm silicone rubber tubing , stoppered with ...
Pagina 237
... cannula is then quickly inserted into the portal vein at the incision site and pushed until the orifice of the cannula has passed the ligature by about 2 mm . The cannula must not be pushed past the first portal branch , in which case ...
... cannula is then quickly inserted into the portal vein at the incision site and pushed until the orifice of the cannula has passed the ligature by about 2 mm . The cannula must not be pushed past the first portal branch , in which case ...
Pagina 321
... cannula into region surrounded by sutures . Tighten lower suture around cannula . 8. Start perfusion pump with HBSSH . Start timing 10 min . Liver will swell . 9. Immediately open chest and cut vena cava above diaphragm . Liver should ...
... cannula into region surrounded by sutures . Tighten lower suture around cannula . 8. Start perfusion pump with HBSSH . Start timing 10 min . Liver will swell . 9. Immediately open chest and cut vena cava above diaphragm . Liver should ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
Parole e frasi comuni
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml