In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 25
... technique , and the late Dr. Margaret R. Murray for support of my early training . Dr. Hanna M. Sobkowicz showed me the method for explanting spinal cord - DRG cultures . Valuable advice on culture technique and establishment of a new ...
... technique , and the late Dr. Margaret R. Murray for support of my early training . Dr. Hanna M. Sobkowicz showed me the method for explanting spinal cord - DRG cultures . Valuable advice on culture technique and establishment of a new ...
Pagina 262
... technique in many laboratories . There is considerable interest and need to apply these techniques to human liver . The development of in vitro systems that can accurately predict the re- sponses of humans has been necessitated in ...
... technique in many laboratories . There is considerable interest and need to apply these techniques to human liver . The development of in vitro systems that can accurately predict the re- sponses of humans has been necessitated in ...
Pagina 348
... techniques . The most commonly employed methods are collagenase digestion of the tissue , either by nonperfusion ( 1,2 ) or ... technique , which involves a one - step perfusion of the tissue with buffer containing collage- nase and ...
... techniques . The most commonly employed methods are collagenase digestion of the tissue , either by nonperfusion ( 1,2 ) or ... technique , which involves a one - step perfusion of the tissue with buffer containing collage- nase and ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml