Microbiological Quality Assurance: A Guide Towards Relevance and Reproducibility of Inocula

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CRC Press, 18 gen 2018 - 317 pagine
Microbiological Quality Assurance: A Guide Towards Relevance and Reproducibility of Inocula sheds light on the difficulties of obtaining results in the test tube that will be reproducible and relevant for a wide variety of tests. This book explores the current state of research in this area and troubleshoots the problems that may be encountered in setting up appropriate cultures. The text divides naturally into three sections-growth conditions, post-growth conditions, and applications. This book serves as a valuable resource for clinical microbiologists, pharmacologists, and anyone doing in vitro experiments.

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Indice

Influence of PostGrowth Procedures on the Properties
Washing and Resuspension of Harvested Cells
1
Reproducibility and Predictivity of Inocula Grown
2
Factors Causing Test Variability
Overview
3

Practical Considerations
3
The Influence of Nutrient Environment During Vegetative Growth
6
In Vitro Models of Biofilms
Conclusions
Sources of Biological Variation and Lack of Inoculum
Preservation by Cooling
FreezePreservation in Practice
FreezeDrying
Lyophilization Damage and Storage Conditions
Alternatives to Vacuum FreezeDrying
4
Inter and Intralaboratory Variability of Test Results
5
Factors Affecting Biological Indicator Performance
QuickResponse Biological Indicators
6
7
InVitro Models for Secondary Screening of Antibiotics
8
9
Index

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