Laboratory Procedures in Clinical Microbiology

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J.A. Washington
Springer Science & Business Media, 6 dic 2012 - 856 pagine
Although there are a number of comprehensive books in clinical micro biology, there remains a need for a manual that can be used in the clinical laboratory to guide the daily performance of its work. Most of the existing publications provide detailed and precise information, for example, by which a microorganism can be characterized and identified beyond any doubt; however, the number of tests involved in this process exceeds the capabilities and resources of most clinical laboratories and are irrelevant for patient care. It is, therefore, necessary in any clinical laboratory to extract from reference manuals, textbooks, and journals those tests and procedures that are to be used to complete the daily workload !is efficiently and accurately as possible. It is also essential in the clinical laboratory to determine, on the basis of the kind of specimen being examined, which microorganisms are clinically relevant and require isolation and identifica tion and which should either be excluded selectively or simply regarded as indigenous Hora and, therefore, not specifically identified. Cost and time limit a laboratory's resources, and priorities must be established for handling the workload. The procedures described in this manual are those selected by our staff for use in the clinical laboratory on the basis of clinical relevance, accuracy, reproducibility, and efficiency. ' Alternative procedures, when considered equivalent on the basis of personal or published experience, have been in cluded where appropriate.
 

Sommario

Collection and Handling of Specimens
1
Specific Guidelines for Specimen Collection
24
References
66
Stains and Wet Mount Preparations
81
References
89
Cultures of Specimens from the Upper Respiratory Tract
100
Cultures of Urine
109
Cultures of Integumentary Infections
115
Serodiagnosis of Fungal Infections
472
References
488
Chlamydiae
493
References
506
References
522
Identification of RNAContaining Viruses
531
Identification of DNAContaining Viruses
576
Reporting of Viral Isolates
598

References
124
2 Identification of Aerobic and Facultatively
133
GramNegative Cocci
157
GramPositive Bacilli
164
GramNegative Bacilli
179
References
241
3 Fluorescent Antibody Procedures
249
Counterimmunoelectrophoresis
268
References
276
Preparation of Antimicrobial Solutions
285
References
306
Isolation of Anaerobic Bacteria
315
Identification of Anaerobic Bacteria
323
Antimicrobial Susceptibility Testing of Anaerobic Bacteria
358
Mycobacteria and Nocardia
365
Antimicrobial Susceptibility Testing
393
References
405
Identification of Yeasts
411
Preliminary Tests
424
Identification of Filamentous Fungi
436
Parasites
609
Procedures Suggested for Use in Examination of Clinical
616
Processing of Stool Specimens
623
Examination for Blood and Tissue Parasites
646
Examination of Specimens Other Than Feces
657
Serology for Parasitic Diseases
664
References
676
Bioassays
684
Liquid Chromatographic Assays
702
References
713
Serum Antibacterial Titer
719
References
726
Routes of LaboratoryAcquired Infections
730
Environmental Control
742
Media and Reagents for Anaerobic Bacteria
776
Media and Reagents for Mycobacteria and Nocardia
783
Media Reagents and Buffers for Chlamydiae
793
References
806
Equipment
837
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