Laboratory Procedures in Clinical MicrobiologyJ.A. Washington Springer Science & Business Media, 6 dic 2012 - 856 pagine Although there are a number of comprehensive books in clinical micro biology, there remains a need for a manual that can be used in the clinical laboratory to guide the daily performance of its work. Most of the existing publications provide detailed and precise information, for example, by which a microorganism can be characterized and identified beyond any doubt; however, the number of tests involved in this process exceeds the capabilities and resources of most clinical laboratories and are irrelevant for patient care. It is, therefore, necessary in any clinical laboratory to extract from reference manuals, textbooks, and journals those tests and procedures that are to be used to complete the daily workload !is efficiently and accurately as possible. It is also essential in the clinical laboratory to determine, on the basis of the kind of specimen being examined, which microorganisms are clinically relevant and require isolation and identifica tion and which should either be excluded selectively or simply regarded as indigenous Hora and, therefore, not specifically identified. Cost and time limit a laboratory's resources, and priorities must be established for handling the workload. The procedures described in this manual are those selected by our staff for use in the clinical laboratory on the basis of clinical relevance, accuracy, reproducibility, and efficiency. ' Alternative procedures, when considered equivalent on the basis of personal or published experience, have been in cluded where appropriate. |
Sommario
1 | |
24 | |
References | 66 |
Stains and Wet Mount Preparations | 81 |
References | 89 |
Cultures of Specimens from the Upper Respiratory Tract | 100 |
Cultures of Urine | 109 |
Cultures of Integumentary Infections | 115 |
Serodiagnosis of Fungal Infections | 472 |
References | 488 |
Chlamydiae | 493 |
References | 506 |
References | 522 |
Identification of RNAContaining Viruses | 531 |
Identification of DNAContaining Viruses | 576 |
Reporting of Viral Isolates | 598 |
References | 124 |
2 Identification of Aerobic and Facultatively | 133 |
GramNegative Cocci | 157 |
GramPositive Bacilli | 164 |
GramNegative Bacilli | 179 |
References | 241 |
3 Fluorescent Antibody Procedures | 249 |
Counterimmunoelectrophoresis | 268 |
References | 276 |
Preparation of Antimicrobial Solutions | 285 |
References | 306 |
Isolation of Anaerobic Bacteria | 315 |
Identification of Anaerobic Bacteria | 323 |
Antimicrobial Susceptibility Testing of Anaerobic Bacteria | 358 |
Mycobacteria and Nocardia | 365 |
Antimicrobial Susceptibility Testing | 393 |
References | 405 |
Identification of Yeasts | 411 |
Preliminary Tests | 424 |
Identification of Filamentous Fungi | 436 |
Parasites | 609 |
Procedures Suggested for Use in Examination of Clinical | 616 |
Processing of Stool Specimens | 623 |
Examination for Blood and Tissue Parasites | 646 |
Examination of Specimens Other Than Feces | 657 |
Serology for Parasitic Diseases | 664 |
References | 676 |
Bioassays | 684 |
Liquid Chromatographic Assays | 702 |
References | 713 |
Serum Antibacterial Titer | 719 |
References | 726 |
Routes of LaboratoryAcquired Infections | 730 |
Environmental Control | 742 |
Media and Reagents for Anaerobic Bacteria | 776 |
Media and Reagents for Mycobacteria and Nocardia | 783 |
Media Reagents and Buffers for Chlamydiae | 793 |
806 | |
Equipment | 837 |
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