A Practical Guide to the Study of Calcium in Living CellsAcademic Press, 25 apr 1994 - 368 pagine A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.
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Sommario
Microelectrode Techniques for Measuring Ca2+i and Ca2+ Fluxes | 91 |
Fluorescence Techniques for Imaging Ca2+i | 153 |
Use of Aequorin for Ca2+i Imaging | 285 |
Index | 359 |
Contents of Previous Volumes | 365 |
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A Practical Guide to the Study of Calcium in Living Cells Richard Nuccitelli Anteprima limitata - 1994 |
Parole e frasi comuni
acid activity aequorin affinity BAPTA binding Biochem Biol biological Biophys buffer Ca”-free Ca”-selective Calcium Green calculated calibration camera cDNA Cell Biology cells cellular changes channels chelators Chem compounds concentration confocal confocal microscopy cyclopiazonic acid cytoplasm cytosolic deblurring described detection detector dichroic mirror digitonin DM-nitrophen effect eggs EGTA electrode emission ester excitation experimental fluorescence free calcium in-focus inhibitors injected intensity intracellular intracellular calcium ion-selective ionic strength ionomycin ionophore laser levels ligand light loaded luminescence measured medium membrane method microinjection micropipette microscope Molecular muscle Nature London neurons nitr oocytes optical organelles out-of-focus patch photolysis photon Physiol pipette pixel potential probe processing protein pump ratio ratiometric receptor recombinant aequorin release response reticulum sarcoplasmic reticulum scanning Section sensitivity SERCA signal silane SNARF-1 solution spatial technique temperature thapsigargin tion Tsien values vibrating vitro voltage wavelength Xenopus oocytes Zucker
Brani popolari
Pagina 357 - Feinberg, AP and Vogelstein, B. (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity.
Pagina 89 - Hanley, MR, and Dawson, AP (1990). Thapsigargin, a tumor promoter, discharges intracellular Ca2+ stores by specific inhibition of the endoplasmic reticulum Ca2+-ATPase.
Pagina 28 - Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells. J. Physiol.
Pagina 337 - Y. (1986). Temporal and spatial dynamics of the periodic increase in intracellular free calcium at fertilization of golden hamster eggs.
Pagina 150 - Perez-Reyes, E., Kim, HS, Lacerda, AE, Home, W., Wei, X., Rampe, D., Campbell, KP, Brown, AM, and Birnbaumer, L. (1989). Induction of calcium currents by the expression of the ai-subunit of the dihydropyridine receptor from skeletal muscle.
Pagina 240 - This work was supported by grants from the National Institutes of Health and the National Science Foundation...
Riferimenti a questo libro
Digital Image Analysis of Microbes: Imaging, Morphometry, Fluorometry and ... M. H. F. Wilkinson,F. Schut Anteprima limitata - 1998 |