RNA Interference Technology: From Basic Science to Drug DevelopmentKrishnarao Appasani Cambridge University Press, 17 gen 2005 RNA Interference (RNAi) technology has rapidly become one of the key methods used in functional genomics. RNAi is used to block the expression of genes and create phenotypes that can potentially yield clues about the function of these genes. In the postgenomic era, the elucidation of the physiological function of genes has become the rate-limiting step in the quest to develop 'gene-based drugs' and RNAi could potentially play a pivotal role in the validation of such novel drugs. In this overview, the basic concepts and applications of RNAi biology are discussed. Leading experts from both academia and industry have contributed to this invaluable reference. The volume is forwarded by Andrew Fire, one of the winners of the 2006 Nobel Prize for the discovery of RNA Interference. |
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RNA Interference Technology: From Basic Science to Drug Development Krishnarao Appasani Anteprima non disponibile - 2011 |
RNA Interference Technology: From Basic Science to Drug Development Krishnarao Appasani Anteprima non disponibile - 2005 |
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Academy of Sciences activity Ambros analysis antisense RNA antisense strand apoptosis assay BACE1 Caenorhabditis elegans cancer cell lines cellular cloned complex culture delivery of siRNA Dicer disease double-stranded RNA Drosophila drug dsRNA effective efficient Elbashir electroporation embryos encoding endogenous enzyme Figure fluorescence functional genomics gene expression gene function gene silencing Genes & Development genetic genome-wide hairpin RNAs Hannon hepatitis high-throughput homologous human identified induced infection inhibition Journal kinase knockdown liver luciferase mammalian cells mediated method mice microRNA miRNAs molecules mouse mRNA mutants National Academy Nature neurons Nucleic Acids nucleotide oligonucleotides phenotypes plants plasmid production promoter protein PTGS regulation replication RISC RNA interference RNA silencing RNAi RNase RNase III Sciences USA screens sequence shRNA siRNA siRNA duplex siRNA expression small interfering RNAs specific studies suppression synthesis target gene target mRNA therapeutic tissue transcription transfected transgenic Tuschl viral vectors virus viruses vitro vivo Zamore
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