Manual of microbiological methods

aerobic agar alcohol amino acids amount anaerobic animals antibody antigen antiserum autoclave bacteria Bacteriol bacteriological Bacteriology biochemical blood broth buffer buffer solutions cells cent centrifugation Chap chart colonies color concentration containing determination dilution dish distilled water drop eggs electrode embryo employed enzyme ethyl ethyl alcohol extract fermentation filter flagella flask fluid fuchsin gelatin glass glucose gram stain gram-negative growth half-cell heat hemagglutination hemolysin hydrogen incubation indicator infection inoculation inoculum isolation laboratory liquid material medium membrane method methylene blue microorganisms mixture NaOH needle nutrient obtained organisms oxidation oxidation-reduction oxygen pathogenicity peptone pipet plate precipitate prepared present procedure production protein pure culture quantitative reaction reagent removed rickettsiae saline salt serum slant slide smears soluble solution species spore Staining schedule standard sterile suitable suspension tap water technic temperature tion tissue titer titration toxin turbidity types usually viral virus viruses Wash