In Situ Hybridization HistochemistryCRC Press, 3 lug 1990 - 224 pagine The goal of this fascinating new book is to review the diversity of methods available to apply in situ hybridization histochemistry (ISHH) to a variety of experimental questions. This work includes topics such as synthesis and use of nick-translated DNA probes for ISHH, synthesis and use of oligomeric DNA probes for ISHH, and synthesis and use of RNA probes for ISHH. These interesting chapters describe the preparation of different radiolabeled probes for ISHH. They also discuss their respective advantages and limitations, and describe current results based on the use of these various probes. Sections of the text highlight low and high resolution autoradiography for ISHH, the use of biotin-labeled probes for ISHH, as well as the use of ISHH in combination with established anatomical techniques. In Situ Hybridization Histochemistry answers all of your questions regarding the quantification of ISHH. It also provides a practical description of typical protocols, both from molecular biology and histology. Investigators will understand and value this useful, powerful tool-whatever their backgrounds might be. |
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2X SSC Acad adult analysis astrocytes autoradiographic axons Baldino Biol biotin biotinylated Brain Res buffer c-fos c-myc calbindin cDNA cellular cerebellar cerebellum chromosome cloning cRNA probes cultures demyelinated detection developmental differentiation Eberwine electron microscopic emulsion encoding enzyme ethanol exon Figure gene expression glial cells granule cells hippocampus Histochem hybridization histochemistry hybridization studies increased induced Insulin labeled lesions localization MBP-mRNA messenger RNA method molecular molecules mRNA mRNA levels mutant mutant mice myelin basic protein Natl nerve growth factor nervous system neural neurons Neurosci NGF receptors nonradioactive probes normal nucleic acid nucleotide nucleus oligodendrocytes oncogene pituitary POMC primer probe binding Proc procedure proto-oncogenes Purkinje cells quantitative radioactive rat brain reaction reeler regions regulation room temperature Schwann cells sequences signal silver grains situ hybridization slides specific activity spinal cord synthesis synthetic oligonucleotide probes target technique tissue sections transcription vasopressin vasopressin mRNA viral