The ACT Cytogenetics Laboratory ManualRevised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
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Pagina 34
Survey results , mitotic arrest - blood cultures ( prophase / prometaphase )
Colcemid concentration ( ug / mL ) Time Comments 0.02 10 min 15 and 30 min
30 min 0.05 10 min 10 min 10 min 10 min 30 min 1 hr 0.06 5 min 0.1 10 min 10
min 1.0 ...
Survey results , mitotic arrest - blood cultures ( prophase / prometaphase )
Colcemid concentration ( ug / mL ) Time Comments 0.02 10 min 15 and 30 min
30 min 0.05 10 min 10 min 10 min 10 min 30 min 1 hr 0.06 5 min 0.1 10 min 10
min 1.0 ...
Pagina 61
Colcemid , last 10 min Velban , 0.01 ug / ml , last hr Colcemid , last 2 hr Colcemid
, 0.06 ug / mL , last 1.5 hr Colcemid , 0.07 ug / mL , 7 min 42 Latos - Bielenska
and Hameisterb lymphocytes EB 2 hr 2.5 x 10-5 mol / L ( MW = 394.3 ) 2 ug / mL
...
Colcemid , last 10 min Velban , 0.01 ug / ml , last hr Colcemid , last 2 hr Colcemid
, 0.06 ug / mL , last 1.5 hr Colcemid , 0.07 ug / mL , 7 min 42 Latos - Bielenska
and Hameisterb lymphocytes EB 2 hr 2.5 x 10-5 mol / L ( MW = 394.3 ) 2 ug / mL
...
Pagina 91
For prometaphase analysis , one flask for actinomycin D , one flask for 30 -
minute Colcemid exposure , and one flask for 1 - hour Colcemid exposure . Ten
mL of medium per flask . Cultures are grown 72 to 96 hours after initiation with
PHA .
For prometaphase analysis , one flask for actinomycin D , one flask for 30 -
minute Colcemid exposure , and one flask for 1 - hour Colcemid exposure . Ten
mL of medium per flask . Cultures are grown 72 to 96 hours after initiation with
PHA .
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Sommario
Peripheral Blood Culture | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
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Informazioni bibliografiche
| Titolo | The ACT Cytogenetics Laboratory Manual |
| Autore | Association of Cytogenetic Technologists |
| Curatore | Margaret J. Barch |
| Edizione | 2, illustrata |
| Editore | Raven Press, 1991 |
| Provenienza dell'originale | la University of Michigan |
| Digitalizzato | 23 lug 2008 |
| ISBN | 0881677744, 9780881677744 |
| Lunghezza | 625 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |