The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
Risultati 1-3 di 90
Pagina 99
... Incubate the cultures at 37 ° C in 5 % CO2 , 5 % O2 , and 90 % N2 for 24 to 48 hours . Harvest with Tecan Robotic Sample Processor . SKIN FIBROBLAST AND OTHER SOLID TISSUE CULTURE METHOD 1. Place the specimen in a a sterile , dry Petri ...
... Incubate the cultures at 37 ° C in 5 % CO2 , 5 % O2 , and 90 % N2 for 24 to 48 hours . Harvest with Tecan Robotic Sample Processor . SKIN FIBROBLAST AND OTHER SOLID TISSUE CULTURE METHOD 1. Place the specimen in a a sterile , dry Petri ...
Pagina 128
... Incubate for 15 to 17 hours at 37 ° C . ( After 17 hours , the cells may begin to die . ) 3. Add 0.1 mL of a 1 : 1 thymidine - hypoxanthine mixture for each 10 mL culture volume . The final concentration of thymidine should be 0.5 x 10 ...
... Incubate for 15 to 17 hours at 37 ° C . ( After 17 hours , the cells may begin to die . ) 3. Add 0.1 mL of a 1 : 1 thymidine - hypoxanthine mixture for each 10 mL culture volume . The final concentration of thymidine should be 0.5 x 10 ...
Pagina 196
... Incubate for 30 minutes at 37 ° C . During this time the outer villus layer ( syncytiotrophoblast ) will begin to separate from the internal portion ( mesen- chymal core ) . 5. After 15 minutes , check for separation of layers using an ...
... Incubate for 30 minutes at 37 ° C . During this time the outer villus layer ( syncytiotrophoblast ) will begin to separate from the internal portion ( mesen- chymal core ) . 5. After 15 minutes , check for separation of layers using an ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
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Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL