The ACT Cytogenetics Laboratory ManualRevised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
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Pagina 274
Rinse . e . Jar 5 : Distilled H2O . Rinse . 2. Dip the slide through the series of
Coplin jars as described . 3. Mount the slide . 4. Check for results under the
microscope . If optimal staining is not achieved , vary the times in trypsin and
stain .
Rinse . e . Jar 5 : Distilled H2O . Rinse . 2. Dip the slide through the series of
Coplin jars as described . 3. Mount the slide . 4. Check for results under the
microscope . If optimal staining is not achieved , vary the times in trypsin and
stain .
Pagina 536
Rinse slides three times ( 3 minutes each ) in 2 x SSC . 9. Incubate the slides in
10 % normal goat serum in blotto for 10 minutes at room temperature . This step
is necessary to block nonspecific antibody - binding sites and extraneous avidin ...
Rinse slides three times ( 3 minutes each ) in 2 x SSC . 9. Incubate the slides in
10 % normal goat serum in blotto for 10 minutes at room temperature . This step
is necessary to block nonspecific antibody - binding sites and extraneous avidin ...
Pagina 593
Rinse bottle three times in repurified water ( tap water run through a deionizer
and organic filter , or through reverse osmosis filtration ) . Fill and empty bottle
completely each time . 3. Drain dry . If bottles are to be stored , dry briefly in oven
on ...
Rinse bottle three times in repurified water ( tap water run through a deionizer
and organic filter , or through reverse osmosis filtration ) . Fill and empty bottle
completely each time . 3. Drain dry . If bottles are to be stored , dry briefly in oven
on ...
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Sommario
Peripheral Blood Culture | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
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Parole e frasi comuni
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Informazioni bibliografiche
| Titolo | The ACT Cytogenetics Laboratory Manual |
| Autore | Association of Cytogenetic Technologists |
| Curatore | Margaret J. Barch |
| Edizione | 2, illustrata |
| Editore | Raven Press, 1991 |
| Provenienza dell'originale | la University of Michigan |
| Digitalizzato | 23 lug 2008 |
| ISBN | 0881677744, 9780881677744 |
| Lunghezza | 625 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |