The ACT Cytogenetics Laboratory ManualRevised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
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Pagina 33
TABLE 1. Survey results , mitotic arrest - amniotic fluids ( dispersal harvest )
Colcemid concentration ( ug / mL ) Time 0.012 3–4 hr 0.02 30 min 2 hr 3 hr 4 hr 2
-4 hr 0.025 3 hr 0.04 3 hr 0.05 30 min 1 hr 2 hr 2-3 hr 10-12 hr 0.06 30-40 min
0.25 2 ...
TABLE 1. Survey results , mitotic arrest - amniotic fluids ( dispersal harvest )
Colcemid concentration ( ug / mL ) Time 0.012 3–4 hr 0.02 30 min 2 hr 3 hr 4 hr 2
-4 hr 0.025 3 hr 0.04 3 hr 0.05 30 min 1 hr 2 hr 2-3 hr 10-12 hr 0.06 30-40 min
0.25 2 ...
Pagina 34
TABLE 4. Survey results , mitotic arrest - blood cultures ( prophase /
prometaphase ) Colcemid concentration ( ug / mL ) Time Comments 0.02 10 min
15 and 30 min 30 min 0.05 10 min 10 min 10 min 10 min 30 min 1 hr 0.06 5 min
0.1 10 min ...
TABLE 4. Survey results , mitotic arrest - blood cultures ( prophase /
prometaphase ) Colcemid concentration ( ug / mL ) Time Comments 0.02 10 min
15 and 30 min 30 min 0.05 10 min 10 min 10 min 10 min 30 min 1 hr 0.06 5 min
0.1 10 min ...
Pagina 60
In all cases , amethopterin concentration is 10-7 mol / L and thymidine
concentration for release is 10-5 mol / L in the culture ( see Table 19 ) . In
addition , 1 laboratory uses FDU ( exact details unavailable ) and thymidine , and
several use ...
In all cases , amethopterin concentration is 10-7 mol / L and thymidine
concentration for release is 10-5 mol / L in the culture ( see Table 19 ) . In
addition , 1 laboratory uses FDU ( exact details unavailable ) and thymidine , and
several use ...
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Sommario
Peripheral Blood Culture | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
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Informazioni bibliografiche
| Titolo | The ACT Cytogenetics Laboratory Manual |
| Autore | Association of Cytogenetic Technologists |
| Curatore | Margaret J. Barch |
| Edizione | 2, illustrata |
| Editore | Raven Press, 1991 |
| Provenienza dell'originale | la University of Michigan |
| Digitalizzato | 23 lug 2008 |
| ISBN | 0881677744, 9780881677744 |
| Lunghezza | 625 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |