The ACT Cytogenetics Laboratory ManualRevised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
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Pagina 231
R Banding by Fluorescence Using Acridine Orange ( AO ) * Phosphate Buffer : 32
mL of 0.07N Na2HPO4 : 12 H2O 68 mL of 0.07 mol / L KH2PO4 Adjust pH to 6.5
by adding 0.07N Na2HPO4 : 12H20 to the solution . Acridine Orange : 0.01 % ...
R Banding by Fluorescence Using Acridine Orange ( AO ) * Phosphate Buffer : 32
mL of 0.07N Na2HPO4 : 12 H2O 68 mL of 0.07 mol / L KH2PO4 Adjust pH to 6.5
by adding 0.07N Na2HPO4 : 12H20 to the solution . Acridine Orange : 0.01 % ...
Pagina 237
Remove the coverslip , and rinse briefly with pH 7.0 buffer . 3. Flood with DAPI
working solution . Coverslip , and incubate the slide in the dark at room
temperature for 5 to 15 minutes . 4. Remove the coverslip , and rinse the slide
briefly with pH ...
Remove the coverslip , and rinse briefly with pH 7.0 buffer . 3. Flood with DAPI
working solution . Coverslip , and incubate the slide in the dark at room
temperature for 5 to 15 minutes . 4. Remove the coverslip , and rinse the slide
briefly with pH ...
Pagina 281
Add 10 mL of stock stain solution to 40 mL buffer for a working solution . Buffer :
Dissolve Gurr's buffer tablets , pH 6.8 , in distilled water , and dilute to 1 L.
METHOD 1. Prepare slides the same day the C - banding procedure will be
performed .
Add 10 mL of stock stain solution to 40 mL buffer for a working solution . Buffer :
Dissolve Gurr's buffer tablets , pH 6.8 , in distilled water , and dilute to 1 L.
METHOD 1. Prepare slides the same day the C - banding procedure will be
performed .
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Sommario
Peripheral Blood Culture | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
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Informazioni bibliografiche
| Titolo | The ACT Cytogenetics Laboratory Manual |
| Autore | Association of Cytogenetic Technologists |
| Curatore | Margaret J. Barch |
| Edizione | 2, illustrata |
| Editore | Raven Press, 1991 |
| Provenienza dell'originale | la University of Michigan |
| Digitalizzato | 23 lug 2008 |
| ISBN | 0881677744, 9780881677744 |
| Lunghezza | 625 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |