The ACT Cytogenetics Laboratory ManualRevised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
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Pagina 112
In a sterile Petri dish containing a small amount of medium supplemented with
fetal alf serum , mince the specimen finely with small scissors or a sharp scalpel if
desired , culture these clumps or disperse them further by pipetting them ...
In a sterile Petri dish containing a small amount of medium supplemented with
fetal alf serum , mince the specimen finely with small scissors or a sharp scalpel if
desired , culture these clumps or disperse them further by pipetting them ...
Pagina 196
Transfer the villi from PBS to a dish containing trypsin . Incubate for 30 minutes at
37 ° C . During this time the outer villus layer ( syncytiotrophoblast ) will begin to
separate from the internal portion ( mesenchymal core ) . 5. After 15 minutes ...
Transfer the villi from PBS to a dish containing trypsin . Incubate for 30 minutes at
37 ° C . During this time the outer villus layer ( syncytiotrophoblast ) will begin to
separate from the internal portion ( mesenchymal core ) . 5. After 15 minutes ...
Pagina 602
Second cell dilution ( should be 2 x 104 cells / 10 mL ) : Add 0.2 mL of 1st cell
dilution to 9.8 ml medium containing 15 ... Add 0.5 mL of final cell suspension (
100 cells per dish ) to each of ten 60 mm plastic culture Petri dishes containing 4
mL ...
Second cell dilution ( should be 2 x 104 cells / 10 mL ) : Add 0.2 mL of 1st cell
dilution to 9.8 ml medium containing 15 ... Add 0.5 mL of final cell suspension (
100 cells per dish ) to each of ten 60 mm plastic culture Petri dishes containing 4
mL ...
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Sommario
Peripheral Blood Culture | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
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Informazioni bibliografiche
| Titolo | The ACT Cytogenetics Laboratory Manual |
| Autore | Association of Cytogenetic Technologists |
| Curatore | Margaret J. Barch |
| Edizione | 2, illustrata |
| Editore | Raven Press, 1991 |
| Provenienza dell'originale | la University of Michigan |
| Digitalizzato | 23 lug 2008 |
| ISBN | 0881677744, 9780881677744 |
| Lunghezza | 625 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |