The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
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Pagina 112
... containing a small amount of medium supplemented with fetal calf serum , mince the specimen finely with small scissors or a sharp scalpel blade ; if desired , culture these clumps or disperse them further by pipetting them repeatedly or ...
... containing a small amount of medium supplemented with fetal calf serum , mince the specimen finely with small scissors or a sharp scalpel blade ; if desired , culture these clumps or disperse them further by pipetting them repeatedly or ...
Pagina 114
... containing a small amount of PBS . 2. Place the cell suspension and clumps in a 125 mL sterile Erlenmeyer flask containing 50 mL pronase solution , and stir the flask slowly for 2 hours on a magnetic stirrer . Add 5 mL of sterile fetal ...
... containing a small amount of PBS . 2. Place the cell suspension and clumps in a 125 mL sterile Erlenmeyer flask containing 50 mL pronase solution , and stir the flask slowly for 2 hours on a magnetic stirrer . Add 5 mL of sterile fetal ...
Pagina 248
... containing template ( * ) strand and a duplicated strand which also will contain thymidine . The other chromatid of each chromosome will consist of a BrdU substituted strand and a duplicated strand containing thymidine . The sister ...
... containing template ( * ) strand and a duplicated strand which also will contain thymidine . The other chromatid of each chromosome will consist of a BrdU substituted strand and a duplicated strand containing thymidine . The sister ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
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Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL