The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
Risultati 1-3 di 85
Pagina 86
... cultures for prophase / prometaphase studies , all synchronized . Cultures are usu- ally initiated at 4:00 P.M. or later in the day . For best results , use polypropylene tubes for resistance to fixative . 2. Incubate cultures ( culture ...
... cultures for prophase / prometaphase studies , all synchronized . Cultures are usu- ally initiated at 4:00 P.M. or later in the day . For best results , use polypropylene tubes for resistance to fixative . 2. Incubate cultures ( culture ...
Pagina 156
... culture of mycoplasma is the only detection method , a species or strain resistant to culture remains undetected . Routine mycoplasma check of all amniotic fluid cultures coming into the laboratory is time - con- suming and is not cost ...
... culture of mycoplasma is the only detection method , a species or strain resistant to culture remains undetected . Routine mycoplasma check of all amniotic fluid cultures coming into the laboratory is time - con- suming and is not cost ...
Pagina 201
... culture medium with phytohemagglutinin , and cultures were incubated at 37 ° C for three days and harvested in the usual manner . Al- though the mitotic index was low , as many as 10 well - spread metaphases were found on one slide ...
... culture medium with phytohemagglutinin , and cultures were incubated at 37 ° C for three days and harvested in the usual manner . Al- though the mitotic index was low , as many as 10 well - spread metaphases were found on one slide ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
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Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL