The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
Risultati 1-3 di 62
Pagina 74
... dish . Allow to stand for 15 minutes . 6. Remove all remaining fixative . Remove slides from dishes , and blow each ... dish containing 3.5 mL of medium . Mix gently , and reincubate the dish for 1.5 hours . 2. Keeping the Petri dish ...
... dish . Allow to stand for 15 minutes . 6. Remove all remaining fixative . Remove slides from dishes , and blow each ... dish containing 3.5 mL of medium . Mix gently , and reincubate the dish for 1.5 hours . 2. Keeping the Petri dish ...
Pagina 78
... dishes flat , remove medium slowly from the edge of the dish using a suction pump . 5. Using a 2 mL Pasteur pipette and bulb , slowly add 1.5 to 2 mL of hypotonic down the edge of the dish . Replace lids , and allow the dish to stand at ...
... dishes flat , remove medium slowly from the edge of the dish using a suction pump . 5. Using a 2 mL Pasteur pipette and bulb , slowly add 1.5 to 2 mL of hypotonic down the edge of the dish . Replace lids , and allow the dish to stand at ...
Pagina 173
... dish for chromosome analysis . CHROMOSOME PREPARATION 1. Add 1 drop of Colcemid ( 5 μg / mL ) from a 25 - gauge needle into the dish . 2. Mix gently , and reincubate the dish for 45 minutes . 3. Keeping the dish flat , remove all the ...
... dish for chromosome analysis . CHROMOSOME PREPARATION 1. Add 1 drop of Colcemid ( 5 μg / mL ) from a 25 - gauge needle into the dish . 2. Mix gently , and reincubate the dish for 45 minutes . 3. Keeping the dish flat , remove all the ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
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Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL