The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
Risultati 1-3 di 38
Pagina 116
... essential and non- essential amino acids , enzymes , hormones , fatty acids , iron , and zinc are also included in an artificial medium . In order to provide for unknown deficiencies , a chem- ically defined medium is usually ...
... essential and non- essential amino acids , enzymes , hormones , fatty acids , iron , and zinc are also included in an artificial medium . In order to provide for unknown deficiencies , a chem- ically defined medium is usually ...
Pagina 118
... essential amino acid that may act as a stimulatory agent as well as an essential nutrient , is usually added just before use because it is very unstable in solution . L- glutamine hydrolyzes to ammonia and glutamic acid , resulting in a ...
... essential amino acid that may act as a stimulatory agent as well as an essential nutrient , is usually added just before use because it is very unstable in solution . L- glutamine hydrolyzes to ammonia and glutamic acid , resulting in a ...
Pagina 240
... essential to have well - spread metaphases with little or no cytoplasm and few or no nuclear remnants around the chromosomes . As previously described , silver - stained slides can be counterstained with Giemsa for easier observation ...
... essential to have well - spread metaphases with little or no cytoplasm and few or no nuclear remnants around the chromosomes . As previously described , silver - stained slides can be counterstained with Giemsa for easier observation ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
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Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL