The ACT Cytogenetics Laboratory ManualRevised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
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Pagina 63
Troubleshooting Harvesting and Slidemaking Possible cause Lan
Troubleshooting ( Continued ) Harvesting and ... patient under anesthesia
Synchronized cells : cells harvested with incorrect release time Cells burst during
harvest procedure .
Troubleshooting Harvesting and Slidemaking Possible cause Lan
Troubleshooting ( Continued ) Harvesting and ... patient under anesthesia
Synchronized cells : cells harvested with incorrect release time Cells burst during
harvest procedure .
Pagina 458
Many technologists believe that multiple harvests should be performed using
different strategies for each harvest on all ... of the first harvest of a given sample
can provide a basis for determining whether modifications of harvesting
procedures ...
Many technologists believe that multiple harvests should be performed using
different strategies for each harvest on all ... of the first harvest of a given sample
can provide a basis for determining whether modifications of harvesting
procedures ...
Pagina 512
On the day of harvest add Colcemid ( 12 drops ) ; BrdU ( .15 mL ) may be added
optionally . Monitor cultures for mitotic index , harvesting between 3 and 5 hours .
Note : BrdU has been shown to suppress fra ( X ) expression when added well ...
On the day of harvest add Colcemid ( 12 drops ) ; BrdU ( .15 mL ) may be added
optionally . Monitor cultures for mitotic index , harvesting between 3 and 5 hours .
Note : BrdU has been shown to suppress fra ( X ) expression when added well ...
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Sommario
Peripheral Blood Culture | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
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abnormalities acid addition allow amniotic analysis appear banding blood bone marrow buffer Cancer cause cell culture cell lines centrifuge changes chemicals chro chromosome clinical Colcemid complete concentration containing count coverslip culture cytogenetic cytoplasmic dark deionized water diluted dish distilled drops exposure filter fixative flask fluid fluorescence fragile frequency fresh gene Genet Giemsa growth harvest human hypotonic increased Incubate involved karyotype labeled laboratory leukemia light material medium metaphase method microscope minutes mol/L mosome needed normal obtained occur patients patterns pellet pipette Place possible preparations procedure processing reference regions Remove reported require Rinse room temperature sample serum short slides solution specific specimen spreading stain step sterile Store studies supernatant suspension Table techniques tissue translocation trypsin tube tumor ug/mL usually volume
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Informazioni bibliografiche
| Titolo | The ACT Cytogenetics Laboratory Manual |
| Autore | Association of Cytogenetic Technologists |
| Curatore | Margaret J. Barch |
| Edizione | 2, illustrata |
| Editore | Raven Press, 1991 |
| Provenienza dell'originale | la University of Michigan |
| Digitalizzato | 23 lug 2008 |
| ISBN | 0881677744, 9780881677744 |
| Lunghezza | 625 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |