The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
Risultati 1-3 di 93
Pagina 26
... incubation . 2. Label each bottle with patient's name , accession number , incubation period , and harvest days . 3. Specimen inoculation a . Newborn to 1 year : prepare buffy coat using 0.75 ml of heparinized peripheral blood by ...
... incubation . 2. Label each bottle with patient's name , accession number , incubation period , and harvest days . 3. Specimen inoculation a . Newborn to 1 year : prepare buffy coat using 0.75 ml of heparinized peripheral blood by ...
Pagina 39
... incubation period . One laboratory uses very short times ( 30-45 seconds ) for adults and longer times ( 2-3 minutes ) for newborns . The 1 laboratory using Ohnuki's hypotonic reports a 2 - hour exposure time . Temperatures for regular ...
... incubation period . One laboratory uses very short times ( 30-45 seconds ) for adults and longer times ( 2-3 minutes ) for newborns . The 1 laboratory using Ohnuki's hypotonic reports a 2 - hour exposure time . Temperatures for regular ...
Pagina 226
... incubation in Ba ( OH ) 2 re- sults in a more complete G - like banding pattern , whereas excessive incubation produces a ghostlike swelling and distortion of the chromosomes . At 60 ° C , the incubation times are somewhat too short to ...
... incubation in Ba ( OH ) 2 re- sults in a more complete G - like banding pattern , whereas excessive incubation produces a ghostlike swelling and distortion of the chromosomes . At 60 ° C , the incubation times are somewhat too short to ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
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Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL