The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
Risultati 1-3 di 86
Pagina 183
... medium two times . If the specimen was not transported in medium with antibiotics ( see the protocol on preparation and use of special antibiotics ) , soak it overnight in complete medium containing , in addition , 1.25 μg / mL ...
... medium two times . If the specimen was not transported in medium with antibiotics ( see the protocol on preparation and use of special antibiotics ) , soak it overnight in complete medium containing , in addition , 1.25 μg / mL ...
Pagina 191
... medium should be 100 units / mL . ( Fifty units / mL final concen- tration in medium is also recommended . ) Streptomycin sulfate : 5 g / 25 mL sterile stock solution ; store frozen in 0.5 mL or appro- priate size ) aliquots ; add 0.05 ...
... medium should be 100 units / mL . ( Fifty units / mL final concen- tration in medium is also recommended . ) Streptomycin sulfate : 5 g / 25 mL sterile stock solution ; store frozen in 0.5 mL or appro- priate size ) aliquots ; add 0.05 ...
Pagina 196
... medium in a 35 mm Petri dish . Transfer the villi from the trypsin solution to the dish containing the medium . Save the trypsin containing mostly outer layer cells and some inner layer cells for step 12 ( optional ) . 7. Mince the ...
... medium in a 35 mm Petri dish . Transfer the villi from the trypsin solution to the dish containing the medium . Save the trypsin containing mostly outer layer cells and some inner layer cells for step 12 ( optional ) . 7. Mince the ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
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Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL