The ACT Cytogenetics Laboratory ManualRevised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
Risultati 1-3 di 94
Pagina 184
As soon as most of the cells are detached ( about 10 minutes ) , rinse them out of
the flask with medium saved during step 2 , adding the cell suspension to the
same centrifuge tube . Be sure to suspend the clumps . 4. Centrifuge at 800 to ...
As soon as most of the cells are detached ( about 10 minutes ) , rinse them out of
the flask with medium saved during step 2 , adding the cell suspension to the
same centrifuge tube . Be sure to suspend the clumps . 4. Centrifuge at 800 to ...
Pagina 272
Clear nail polish : Store at room temperature in a reagent cabinet . Preparation of
McIlvaine's buffer , pH 7.0 METHOD 1. Stain the slide in 0.5 % quinacrine for 2.5
to 3.5 minutes . The timing depends on the age of the quinacrine solution . 2.
Clear nail polish : Store at room temperature in a reagent cabinet . Preparation of
McIlvaine's buffer , pH 7.0 METHOD 1. Stain the slide in 0.5 % quinacrine for 2.5
to 3.5 minutes . The timing depends on the age of the quinacrine solution . 2.
Pagina 293
At 71.25 hours , add 0.05 mL of 10 ug / mL Colcemid , mix well , and return the
tube to the incubator for 45 minutes . If the blood does not settle in 2 hours it may
be transferred to a sterile centrifuge tube and spun at room temperature at 800 ...
At 71.25 hours , add 0.05 mL of 10 ug / mL Colcemid , mix well , and return the
tube to the incubator for 45 minutes . If the blood does not settle in 2 hours it may
be transferred to a sterile centrifuge tube and spun at room temperature at 800 ...
Cosa dicono le persone - Scrivi una recensione
Nessuna recensione trovata nei soliti posti.
Sommario
Peripheral Blood Culture | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
14 sezioni non visualizzate
Parole e frasi comuni
abnormalities acid addition allow amniotic analysis appear banding blood bone marrow buffer Cancer cause cell culture cell lines centrifuge changes chemicals chro chromosome clinical Colcemid complete concentration containing count coverslip culture cytogenetic cytoplasmic dark deionized water diluted dish distilled drops exposure filter fixative flask fluid fluorescence fragile frequency fresh gene Genet Giemsa growth harvest human hypotonic increased Incubate involved karyotype labeled laboratory leukemia light material medium metaphase method microscope minutes mol/L mosome needed normal obtained occur patients patterns pellet pipette Place possible preparations procedure processing reference regions Remove reported require Rinse room temperature sample serum short slides solution specific specimen spreading stain step sterile Store studies supernatant suspension Table techniques tissue translocation trypsin tube tumor ug/mL usually volume
Riferimenti a questo libro
Informazioni bibliografiche
| Titolo | The ACT Cytogenetics Laboratory Manual |
| Autore | Association of Cytogenetic Technologists |
| Curatore | Margaret J. Barch |
| Edizione | 2, illustrata |
| Editore | Raven Press, 1991 |
| Provenienza dell'originale | la University of Michigan |
| Digitalizzato | 23 lug 2008 |
| ISBN | 0881677744, 9780881677744 |
| Lunghezza | 625 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |