The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
Risultati 1-3 di 62
Pagina 29
... needed is ( 20 × 10 ° cells ) / ( 8.0 × 10 cells / mL ) = 2.5 mL plasma ( 40 × 106 cells ) / ( 8.0 × 10 cells / mL ) = 5.0 mL In this example , 2.5 mL plasma would be needed for a minimum cell concentration and 5.0 mL would be needed ...
... needed is ( 20 × 10 ° cells ) / ( 8.0 × 10 cells / mL ) = 2.5 mL plasma ( 40 × 106 cells ) / ( 8.0 × 10 cells / mL ) = 5.0 mL In this example , 2.5 mL plasma would be needed for a minimum cell concentration and 5.0 mL would be needed ...
Pagina 154
... needed and is usually de- termined empirically ( see text ) minimum of 15 ; more as indicated ( see text ) minimum ... needed ( see text ) try for 100 % ( see text ) depends on patient's medical needs ( see text ) buffer system is used ...
... needed and is usually de- termined empirically ( see text ) minimum of 15 ; more as indicated ( see text ) minimum ... needed ( see text ) try for 100 % ( see text ) depends on patient's medical needs ( see text ) buffer system is used ...
Pagina 592
... needed if particulate material or serum is present . 3. If filter holders and accessory parts are reused , they should be washed immediately and rinsed for any items coming in contact with cells ( see the next section on washing of ...
... needed if particulate material or serum is present . 3. If filter holders and accessory parts are reused , they should be washed immediately and rinsed for any items coming in contact with cells ( see the next section on washing of ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
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Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL