The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
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Pagina 155
... oxygen , which is toxic above the con- centration normally in air ) . Some media equilibrate with 10 % CO2 . If cells are shipped from another lab- oratory , it is important to know what concentration of CO2 is needed for the medium on ...
... oxygen , which is toxic above the con- centration normally in air ) . Some media equilibrate with 10 % CO2 . If cells are shipped from another lab- oratory , it is important to know what concentration of CO2 is needed for the medium on ...
Pagina 164
... oxygen supply enhances growth in culture of human mid - trimester am- niotic fluid cells . Hum Genet 1983 ; 64 : 334 . 8. Brambati B , Oldrini A , Ferrazzi E , Lanzani A. Chorionic villus sampling : an analysis of the obstetric ...
... oxygen supply enhances growth in culture of human mid - trimester am- niotic fluid cells . Hum Genet 1983 ; 64 : 334 . 8. Brambati B , Oldrini A , Ferrazzi E , Lanzani A. Chorionic villus sampling : an analysis of the obstetric ...
Pagina 313
... Oxygen weakens developer ; therefore , the flexible containers are especially useful in limiting contact with air . However , polyethylene has its disadvantages : It absorbs chemicals and will even- tually crack if air is squeezed out ...
... Oxygen weakens developer ; therefore , the flexible containers are especially useful in limiting contact with air . However , polyethylene has its disadvantages : It absorbs chemicals and will even- tually crack if air is squeezed out ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
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Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL