The ACT Cytogenetics Laboratory ManualRevised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
Risultati 1-3 di 69
Pagina 83
Aspirate the supernatant from the medium tube , and add 5 mL hypotonic solution
to the tube . Aspirate the supernatant from the trypsin tube , add 0.5 mL hypotonic
solution to the tube , and resuspend the pellet . Add the contents of the trypsin ...
Aspirate the supernatant from the medium tube , and add 5 mL hypotonic solution
to the tube . Aspirate the supernatant from the trypsin tube , add 0.5 mL hypotonic
solution to the tube , and resuspend the pellet . Add the contents of the trypsin ...
Pagina 184
Aspirate most of the supernatant , being careful not to disturb the cell pellet . 6.
Gently add 5 to 10 mL of hypotonic solution ( depending on pellet size ) and
resuspend it thoroughly ( by pushing it out with a siliconized Pasteur pipette ) .
Incubate ...
Aspirate most of the supernatant , being careful not to disturb the cell pellet . 6.
Gently add 5 to 10 mL of hypotonic solution ( depending on pellet size ) and
resuspend it thoroughly ( by pushing it out with a siliconized Pasteur pipette ) .
Incubate ...
Pagina 191
Using sterile technique , add 20 mL of sterile 3x distilled water to a lyophilized
pellet . Swirl the vial to dissolve the pellet . Aliquot 1.2 mL ( or the appropriate
amount ) of amphotericin solution per sterile polystyrene tube , and cap . Label ,
date ...
Using sterile technique , add 20 mL of sterile 3x distilled water to a lyophilized
pellet . Swirl the vial to dissolve the pellet . Aliquot 1.2 mL ( or the appropriate
amount ) of amphotericin solution per sterile polystyrene tube , and cap . Label ,
date ...
Cosa dicono le persone - Scrivi una recensione
Nessuna recensione trovata nei soliti posti.
Sommario
Peripheral Blood Culture | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
14 sezioni non visualizzate
Parole e frasi comuni
abnormalities acid addition allow amniotic analysis appear banding blood bone marrow buffer Cancer cause cell culture cell lines centrifuge changes chemicals chro chromosome clinical Colcemid complete concentration containing count coverslip culture cytogenetic cytoplasmic dark deionized water diluted dish distilled drops exposure filter fixative flask fluid fluorescence fragile frequency fresh gene Genet Giemsa growth harvest human hypotonic increased Incubate involved karyotype labeled laboratory leukemia light material medium metaphase method microscope minutes mol/L mosome needed normal obtained occur patients patterns pellet pipette Place possible preparations procedure processing reference regions Remove reported require Rinse room temperature sample serum short slides solution specific specimen spreading stain step sterile Store studies supernatant suspension Table techniques tissue translocation trypsin tube tumor ug/mL usually volume