The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
Risultati 1-3 di 61
Pagina 58
... periods of the cell cycle are G , ( gap one ) , the interval before chromosome replication ; S ( synthesis ) , the period of DNA synthesis ( replication ) ; G2 ( gap two ) , the interval after synthesis ; and M ( mitosis ) , the period ...
... periods of the cell cycle are G , ( gap one ) , the interval before chromosome replication ; S ( synthesis ) , the period of DNA synthesis ( replication ) ; G2 ( gap two ) , the interval after synthesis ; and M ( mitosis ) , the period ...
Pagina 93
... period of time , add 4 to 6 drops of actinomycin D to each culture bottle in semidarkness . Incubate the cultures for 5 minutes . 2. Add 5 to 6 drops of Colcemid to each culture bottle . Incubate for 30 minutes . 3. Pour the contents of ...
... period of time , add 4 to 6 drops of actinomycin D to each culture bottle in semidarkness . Incubate the cultures for 5 minutes . 2. Add 5 to 6 drops of Colcemid to each culture bottle . Incubate for 30 minutes . 3. Pour the contents of ...
Pagina 146
... period of DNA repair ; the period when every chromosome has doubled and consists of two identical elements known as sister chromatids M the period of mitotic division Cell line cells that have grown for extended periods in vitro ; cells ...
... period of DNA repair ; the period when every chromosome has doubled and consists of two identical elements known as sister chromatids M the period of mitotic division Cell line cells that have grown for extended periods in vitro ; cells ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
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Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL