The ACT Cytogenetics Laboratory ManualRevised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
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Pagina 156
Slide chambers or coverslips for in situ processing may go longer before a
medium change is made , but addition of medium is usually done earlier . A
complete medium change with a rinse first may be indicated if debris or red blood
cells are ...
Slide chambers or coverslips for in situ processing may go longer before a
medium change is made , but addition of medium is usually done earlier . A
complete medium change with a rinse first may be indicated if debris or red blood
cells are ...
Pagina 181
9. Begin to check coverslips on day five or six . Process them when small
colonies ( 50– 200 cells ) are present . If the coverslips are not ready for
processing , perform medium changes twice weekly . PROCESSING * 1. Add 1
drop of Colcemid ...
9. Begin to check coverslips on day five or six . Process them when small
colonies ( 50– 200 cells ) are present . If the coverslips are not ready for
processing , perform medium changes twice weekly . PROCESSING * 1. Add 1
drop of Colcemid ...
Pagina 195
Direct processing can be performed without overnight incubation if time permits .
8. Use the remaining villi for the long - term culture . Incubate these villi in Chang
C medium at 37.5 ° C and 5 % CO2 until long - term cultures can be set up .
Direct processing can be performed without overnight incubation if time permits .
8. Use the remaining villi for the long - term culture . Incubate these villi in Chang
C medium at 37.5 ° C and 5 % CO2 until long - term cultures can be set up .
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Sommario
Peripheral Blood Culture | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
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Parole e frasi comuni
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Informazioni bibliografiche
| Titolo | The ACT Cytogenetics Laboratory Manual |
| Autore | Association of Cytogenetic Technologists |
| Curatore | Margaret J. Barch |
| Edizione | 2, illustrata |
| Editore | Raven Press, 1991 |
| Provenienza dell'originale | la University of Michigan |
| Digitalizzato | 23 lug 2008 |
| ISBN | 0881677744, 9780881677744 |
| Lunghezza | 625 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |