The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
Risultati 1-3 di 75
Pagina 156
... processing , and the number of cultures set up and maintained . If special culture morphology is being studied , such as the presence of rapidly adhering cells in cases of neural tube defect , culture morphology will need to be ...
... processing , and the number of cultures set up and maintained . If special culture morphology is being studied , such as the presence of rapidly adhering cells in cases of neural tube defect , culture morphology will need to be ...
Pagina 181
... processing , perform medium changes twice weekly . PROCESSING * 1. Add 1 drop of Colcemid ( 10 μg / mL ) from a 28 - gauge needle into the Petri dish . Mix gently by swirling , and return the dish to an incubator for 1 hour . 2. Using a ...
... processing , perform medium changes twice weekly . PROCESSING * 1. Add 1 drop of Colcemid ( 10 μg / mL ) from a 28 - gauge needle into the Petri dish . Mix gently by swirling , and return the dish to an incubator for 1 hour . 2. Using a ...
Pagina 195
... PROCESSING 1. Using sterile technique add 0.025 mL of Colcemid ( 10 μg / mL ) to 35 mm dish containing villi for direct processing . ( The 0.025 mL is 6 drops from an insulin syringe with a 28 - gauge needle . ) 2. Incubate at 37 ° C ...
... PROCESSING 1. Using sterile technique add 0.025 mL of Colcemid ( 10 μg / mL ) to 35 mm dish containing villi for direct processing . ( The 0.025 mL is 6 drops from an insulin syringe with a 28 - gauge needle . ) 2. Incubate at 37 ° C ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
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Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL