The ACT Cytogenetics Laboratory ManualRevised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
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Pagina 39
0.075 mol / L KCI : 0.8 % NaCitrate 20 37 ° C a Room temperature 20 Thirteen
laboratories reported use of room temperature hypotonic , 13 use 37 ° C , and 2
laboratories prewarm the hypotonic but incubate cells at room temperature .
0.075 mol / L KCI : 0.8 % NaCitrate 20 37 ° C a Room temperature 20 Thirteen
laboratories reported use of room temperature hypotonic , 13 use 37 ° C , and 2
laboratories prewarm the hypotonic but incubate cells at room temperature .
Pagina 293
At 71.25 hours , add 0.05 mL of 10 ug / mL Colcemid , mix well , and return the
tube to the incubator for 45 minutes . If the blood does not settle in 2 hours it may
be transferred to a sterile centrifuge tube and spun at room temperature at 800 ...
At 71.25 hours , add 0.05 mL of 10 ug / mL Colcemid , mix well , and return the
tube to the incubator for 45 minutes . If the blood does not settle in 2 hours it may
be transferred to a sterile centrifuge tube and spun at room temperature at 800 ...
Pagina 536
Incubate the slides in 10 % normal goat serum in blotto for 10 minutes at room
temperature . This step is necessary to block nonspecific antibody - binding sites
and extraneous avidin - binding sites . 10. Rinse slides twice ( 2 minutes each ) in
...
Incubate the slides in 10 % normal goat serum in blotto for 10 minutes at room
temperature . This step is necessary to block nonspecific antibody - binding sites
and extraneous avidin - binding sites . 10. Rinse slides twice ( 2 minutes each ) in
...
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Sommario
Peripheral Blood Culture | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
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Parole e frasi comuni
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Informazioni bibliografiche
| Titolo | The ACT Cytogenetics Laboratory Manual |
| Autore | Association of Cytogenetic Technologists |
| Curatore | Margaret J. Barch |
| Edizione | 2, illustrata |
| Editore | Raven Press, 1991 |
| Provenienza dell'originale | la University of Michigan |
| Digitalizzato | 23 lug 2008 |
| ISBN | 0881677744, 9780881677744 |
| Lunghezza | 625 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |