The ACT Cytogenetics Laboratory ManualRaven Press, 1991 - 625 pagine Revised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
Dall'interno del libro
Risultati 1-3 di 94
Pagina 39
... Room temperature 222222 warm → RT RT RT 37 ° C RT 37 ° C to 5 minutes ( 3 laboratories ) to 42 minutes ( 1 labora- tory ) for prometaphase bloods . Note that the labora- tory reporting the use of 0 minutes in hypotonic cen- trifuges ...
... Room temperature 222222 warm → RT RT RT 37 ° C RT 37 ° C to 5 minutes ( 3 laboratories ) to 42 minutes ( 1 labora- tory ) for prometaphase bloods . Note that the labora- tory reporting the use of 0 minutes in hypotonic cen- trifuges ...
Pagina 51
... Temperature The following temperatures can affect results : the temperature of the air in the room ; the temperature of the slides and the water with which they are coated ; and the temperature at which the slide is allowed to dry ...
... Temperature The following temperatures can affect results : the temperature of the air in the room ; the temperature of the slides and the water with which they are coated ; and the temperature at which the slide is allowed to dry ...
Pagina 293
... room temperature at 800 RPM for 7 minutes . If 0.1 mL of plasma is present on top of the specimen after centrifugation , this may be removed aseptically and cultures may be established as described in step 2. If a large amount of plasma ...
... room temperature at 800 RPM for 7 minutes . If 0.1 mL of plasma is present on top of the specimen after centrifugation , this may be removed aseptically and cultures may be established as described in step 2. If a large amount of plasma ...
Sommario
Contributors | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
Copyright | |
14 sezioni non visualizzate
Parole e frasi comuni
20 minutes acetic acid Actinomycin amethopterin amniotic fluid blood cultures bone marrow BrdU buffer Cancer Genet Cytogenet cell culture cell lines cell pellet cell suspension centrifuge tube centromere chemicals chro chromatin chromo chromosomal abnormalities clinical Colcemid concentration containing coverslip culture medium cytogenetic deletion diagnosis diluted distilled water drops enzyme exposure fetal fibroblasts film filter flask fluorescence fra(X fragile site fragile sites G banding gene GIBCO growth harvest heterochromatin Hum Genet human chromosomes hybridization hypotonic solution Incubate karyotype labeled laboratory leukemia long arm lymphocytes metaphase methanol method microscope mitotic mol/L KCl mosaicism mosome NaCitrate normal Pasteur pipette patients patterns peripheral blood Petri dish prenatal preparations procedure prometaphase protein quinacrine REAGENTS regions resuspend Rinse room temperature RPMI sample serum short arm slides sodium solid tumors specimen sterile Store studies supernatant syndrome techniques thymidine tissue culture translocation trisomy trypsin usually X chromosome µg/mL