The ACT Cytogenetics Laboratory ManualRevised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
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Pagina 82
EVALUATION AND TRANSPORT Evaluate the sample at the time of aspiration
on a stereomicroscope at 10 to 20x magnification . Transfer the pieces of villus
with sterile forceps from the aspiration medium into a sterile 15 mL centrifuge
tube .
EVALUATION AND TRANSPORT Evaluate the sample at the time of aspiration
on a stereomicroscope at 10 to 20x magnification . Transfer the pieces of villus
with sterile forceps from the aspiration medium into a sterile 15 mL centrifuge
tube .
Pagina 425
fuge the sample at 800 RPM ( 150 x g ) and remove the buffy coat . This
procedure removes a significant fraction of the red blood cells and concentrates
the number of cells in the sample . Preparing a buffy coat is thus particularly
important for ...
fuge the sample at 800 RPM ( 150 x g ) and remove the buffy coat . This
procedure removes a significant fraction of the red blood cells and concentrates
the number of cells in the sample . Preparing a buffy coat is thus particularly
important for ...
Pagina 426
Do not score mature polymorphonuclear leukocytes . 3. Calculate the number of
cells contained in the sample using the following formula : Multiply the number of
cells in 10 squares by 103 ( the hemacytometer factor that converts mm ' to mL ) ...
Do not score mature polymorphonuclear leukocytes . 3. Calculate the number of
cells contained in the sample using the following formula : Multiply the number of
cells in 10 squares by 103 ( the hemacytometer factor that converts mm ' to mL ) ...
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Sommario
Peripheral Blood Culture | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
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Informazioni bibliografiche
| Titolo | The ACT Cytogenetics Laboratory Manual |
| Autore | Association of Cytogenetic Technologists |
| Curatore | Margaret J. Barch |
| Edizione | 2, illustrata |
| Editore | Raven Press, 1991 |
| Provenienza dell'originale | la University of Michigan |
| Digitalizzato | 23 lug 2008 |
| ISBN | 0881677744, 9780881677744 |
| Lunghezza | 625 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |