The ACT Cytogenetics Laboratory ManualRevised and expanded to cover advanced instrumentation techniques. There are three separate chapters on peripheral blood culture, continuous cell lines and prenatal diagnosis and culture and new chapters on solid tumours, fragile sites, and molecular cytogenetics. |
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Pagina 21
Complete medium ( with serum ) has a shelf life of four weeks at 4 ° C . The most
commonly used media ( besides the commercial chromosome kits made by
GIBCO and Difco ) for blood culturing are Eagle's MEM ( Minimum Essential
Medium ) ...
Complete medium ( with serum ) has a shelf life of four weeks at 4 ° C . The most
commonly used media ( besides the commercial chromosome kits made by
GIBCO and Difco ) for blood culturing are Eagle's MEM ( Minimum Essential
Medium ) ...
Pagina 502
Culture Period Serum Serum is a cell growth - promoting component added to
most defined media for cell culture purposes . Its overall concentration in a
medium is usually a function of the type of cells being grown ; concentrations
roughly ...
Culture Period Serum Serum is a cell growth - promoting component added to
most defined media for cell culture purposes . Its overall concentration in a
medium is usually a function of the type of cells being grown ; concentrations
roughly ...
Pagina 602
4. Using sterile technique , make up a series of cell dilutions using 10 mL plastic
culture tubes and a 1 mL plastic disposable pipette . Suspend cells in the
medium with serum to be tested . Be sure cells are well suspended before each
dilution ...
4. Using sterile technique , make up a series of cell dilutions using 10 mL plastic
culture tubes and a 1 mL plastic disposable pipette . Suspend cells in the
medium with serum to be tested . Be sure cells are well suspended before each
dilution ...
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Sommario
Peripheral Blood Culture | 17 |
Harvesting SlideMaking and Chromosome Elongation Techniques | 31 |
Cytogenetic Analysis of Continuous Cell Lines | 107 |
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Informazioni bibliografiche
| Titolo | The ACT Cytogenetics Laboratory Manual |
| Autore | Association of Cytogenetic Technologists |
| Curatore | Margaret J. Barch |
| Edizione | 2, illustrata |
| Editore | Raven Press, 1991 |
| Provenienza dell'originale | la University of Michigan |
| Digitalizzato | 23 lug 2008 |
| ISBN | 0881677744, 9780881677744 |
| Lunghezza | 625 pagine |
| Esporta citazione | BiBTeX EndNote RefMan |